Rapid 3’-End ssRNA Labeling & Purification Kit

Description

The Rapid 3′-End ssRNA Labeling and Purification Kit enables efficient, covalent attachment of fluorescent dyes, biotin, DBCO, or azide functional groups to the 3’ end of single-stranded RNA, including unstructured RNA oligonucleotides, siRNA and mRNA.

A standard reaction can label up to 400 pmol short RNA (18 ~ 250 nt) or 30 µg long RNA (≥ 250 nt), with a typical labeling efficiency of 90-100%. The labeled RNA product can be purified using the provided silica column, achieving a recovery rate of approximately 60% and 80% for short and long RNA respectively.

The resulting 3′‑labeled ssRNA can be used in a broad range of applications, including:

Key Features

• Efficient 3′-end labeling – Enables covalent attachment of fluorescent dyes, biotin, DBCO, or azide groups specifically to the 3′ end of ssRNA.
• Broad RNA compatibility – Works with unstructured RNA oligonucleotides, siRNA, and mRNA across a wide size range (18 nt to full-length transcripts).
• High reaction capacity – A single standard reaction can label up to 400 pmol of short RNA (18–250 nt) or 30 µg of long RNA (≥ 250 nt).
• Integrated purification – Includes a silica spin column for rapid cleanup, with ~60% recovery for short RNA and ~80% for long RNA.

Applications

This kit enables a broad spectrum of applications in biological research, diagnostics, and analytical workflows. The 3’-end labeled single-stranded RNA generated can be utilized for:

• RNA tracking, cell uptake analysis, and imaging.
• Affinity purification, pull-down assays, and protein interaction analysis.
• Click chemistry for conjugation with molecules, probes, or delivery carriers.
• Enhances membrane affinity and delivery efficiency.
• Liver-targeted delivery, especially for hepatocyte-related applications