Rapid eOligoLinker Antibody Conjugation Kit

Description

The Rapid eOligoLinker Antibody Conjugation Kit is designed to enable speed, high-quality antibody-oligonucleotide conjugation. This kit works directly with ssDNA (20–80 nt) and forms a stable 3’‑terminal conjugate with antibody for forward applications.

A standard reaction can label Azide functional group in up to 15 nmol of ssDNA (20–80 nt) with a typical recovery of 85–95% after purification. Subsequent maleimide-modified reaction followed by ethanol precipitation, typically yields total recoveries of 70–90%. The separation of labeled ssDNA from unreacted components is achieved using a straightforward spin-column purification method. This procedure effectively removes contaminants and provides clean, pure labeled ssDNA that is ready for immediate use.

The resulting 3′‑labeled ssDNA can be used in a broad range of applications, including:

Key Features

• Direct 3’-end modification – Works with standard ssDNA (20–80 nt) without requiring special nucleotides.
• High yield and recovery – Typical 60–80% recovery from 15 nmol input.
• eOligoLinker stability – Ensure eOligoLinker activity via two-step
• Preserved activity – Maintains antibody binding function after conjugation.
• Simple workflow – Efficient labeling and purification with a quick spin-column cleanup.

Applications

This kit enables a broad spectrum of applications in biological research, diagnostics, and analytical workflows. The antibody–oligonucleotide conjugates generated can be utilized for:

• Antibody-eoligolinker conjugates for advanced spatial transcriptomics and in situ
• Development of next-generation immuno-PCR and proximity-based assays requiring DNA-tagged antibodies.
• Single-cell and spatial biology workflows involving oligonucleotide-barcoded antibodies.

Paired with qPCR/dPCR readout designs, it powers precise detection via Immuno-PCR.