FPG (Formamidopyrimidine-DNA Glycosylase)

Description

Formamidopyrimidine-DNA Glycosylase (FPG) is a DNA repair enzyme that recognizes and excises oxidized purines, such as formamidopyrimidines (FapyA, FapyG) and 8-oxoguanine, from double-stranded or single-stranded DNA. After base removal, FPG cleaves the DNA backbone at the resulting AP site via its associated AP lyase activity, generating a single-strand break with 3’- and 5’-phosphate termini. This dual activity makes FPG a valuable tool for studying oxidative DNA damage and repair, as well as for damage-specific DNA cleavage in molecular biology applications.

Features

• Dual Activity – DNA glycosylase + AP lyase activities in a single enzyme.
• Specific for Oxidized Purines – Efficiently excises FapyA, FapyG, and 8-oxoguanine lesions.
• Broad Substrate Range – Active on dsDNA, ssDNA, and bubble DNA structures.
• Useful for Oxidative Damage Detection – Generates site-specific strand breaks at damaged bases.

Applications

• Detection and quantitation of oxidative DNA damage
• Mapping 8-oxoguanine and Fapy lesions in genomic DNA
• Oxidative damage-specific DNA cleavage prior to sequencing or PCR
• Preparation of DNA for strand break labeling assays (e.g., comet assay, ligation-mediated PCR)
• In vitro DNA repair studies

Unit Definition

One unit is defined as the amount of enzyme required to cleave 10 pmol of oligonucleotide duplex containing a single 8-oxoguanine base paired with a cytosine in 1 hour at 37°C.

Storage Temperature

-20°C for optimal long-term stability

Storage Buffer

25 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5 mM DTT, 0.5 mM EDTA, 50% Glycerol